Answer
$x^2-8x^4+16x^6$
Work Step by Step
Using $(a+b)^2=a^2+2ab+b^2$ or the square of a binomial, the given expression, $
\left( x-4x^3 \right)^2
$, is equivalent to
\begin{array}{l}\require{cancel}
(x)^2+2(x)(-4x^3)+(-4x^3)^2
\\\\=
x^2-8x^4+16x^6
.\end{array}