#### Answer

The operation is not defined because order is different.

#### Work Step by Step

Consider,
$A-C=\left[ \begin{array}{*{35}{l}}
4 & 0 \\
-3 & 5 \\
0 & 1 \\
\end{array} \right]-\left[ \begin{matrix}
1 & -1 \\
-1 & 1 \\
\end{matrix} \right]$
This subtraction cannot be defined because the matrix is of the size $2\times 3$ and matrix $C$ is of the
size $2\times 2$. For subtraction, the size of both matrices must be the same.
Here, the size of the matrix $A$ is different from the size of the matrix $C$. Therefore, the operation of subtraction is not defined.