Marguerite was married twice, first to Charles IV of Alençon, but this marriage was childless.
Her next marriage was to Henry II of Navarre. The children of Marguerite and Henry were:
- Jeanne III of Navarre (16 November 1528– 9 June 1572),[8] the mother of the future Henry IV of France, also known as Henry III of Navarre. She became Queen regnant of Navarre from 1555 to 1572. She was the wife of Antoine de Bourbon, Duke of Vendôme, and mother of Henry of Bourbon, who became King of Navarre and also of France as the first Bourbon king. She was the acknowledged spiritual and political leader of the French Huguenot movement.[9]
- Jean (7 July 1530- 25 December 1530), who died as an infant
