- Theseus, Duke of Athens
- Palamon, nephew of the King of Thebes
- Arcite, nephew of the King of Thebes
- Pirithous, an Athenian general
- Artesius, an Athenian captain
- Valerius, a noble of Thebes
- Six Knights
- A Herald
- A Jailer
- Wooer of the jailer's daughter
- A Doctor
- Brother of the jailer
- Friends of the jailer
- A Gentleman
- Gerrold, a schoolmaster
- Hippolyta, wife of Theseus
- Emilia, her sister
- Three Queens
- Jailer's Daughter
- Emilia's Servant
- Country Wenches and Women personating Hymen, Boy
- A Laborer
- Countrymen, Messengers
- A Man personating Hymen, Boy
- Executioners, Guards, Soldiers, Attendants
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