Answer
$$W=-2 \mathrm{e}^{x} \mathrm{e}^{-x}$$
Work Step by Step
The Wronskian of $\left\{e^{x}, e^{-x}\right\}$ is given by
$$W=\left|\begin{array}{cc}{e^{x}} & {e^{-x}} \\ {e^{x}} & {-e^{-x}}\end{array}\right|=-2 \mathrm{e}^{x} \mathrm{e}^{-x}$$