- Duncan – king of Scotland
- Malcolm – Duncan's elder son
- Donalbain – Duncan's younger son
- Macbeth – a general in the army of King Duncan; originally Thane of Glamis, then Thane of Cawdor, and later king of Scotland
- Lady Macbeth – Macbeth's wife, and later queen of Scotland
- Banquo – Macbeth's friend and a general in the army of King Duncan
- Fleance – Banquo's son
- Macduff – Thane of Fife
- Lady Macduff – Macduff's wife
- Macduff's son
- Ross, Lennox, Angus, Menteith, Caithness – Scottish thanes
- Siward – general of the English forces
- Young Siward – Siward's son
- Seyton – Macbeth's armourer
- Hecate – queen of the witches
- Three Witches
- Captain – in the Scottish army
- Murderers – employed by Macbeth
- Third Murderer
- Porter – gatekeeper at Macbeth's home
- Doctor – Lady Macbeth's doctor
- Doctor – at the English court
- Gentlewoman – Lady Macbeth's caretaker
- Lord – opposed to Macbeth
- First Apparition – armed head
- Second Apparition – bloody child
- Third Apparition – crowned child
- Attendants, Messengers, Servants, Soldiers
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