- Mariam (Mariamne I) – Queen of Judaea and Herod's second wife
- Herod the Great – King of Judaea
- Salome – Herod's sister
- Pheroras – Herod's brother
- Alexandra – Mariam's mother
- Silleus – Prince of Arabia and Salome's lover
- Constabarus – Salome's husband
- Graphina – Pheroras' lover
- Doris – Herod's first wife and Antipater's mother
- Antipater – Herod and Doris' son
- Ananell – High Priest
- Sohemus – Herod's counselor
- Nuntio – Messenger
- Babas' First Son – Hidden from Herod by Constabarus
- Babas' Second Son – Hidden from Herod by Constabarus
- Chorus – A company of Jews
- Butler to Herod
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