- Thorowgood, a London merchant
- George Barnwell, apprentice to Thorowgood
- Trueman, apprentice to Thorowgood
- Blunt, servant of Millwood
- Barnwell, uncle of George Barnwell
- Maria, daughter of Thorowgood
- Sarah Millwood, a lady of pleasure
- Lucy, servant of Millwood
- Officers with their attendants, keeper of the prison, executioner and footmen
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