Chapter 8 - Gene Transfer and Genetic Engineering - Critical Thinking Questions - Page 239: 2

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Cut the plasmid with a specific restriction enzyme. Cut a DNA sequence with the same enzyme. This will result in a DNA fragment with sticky ends that will anneal with the plasmid spontaneously. Mix the DNA and the plasmid. Use a ligase enzyme to join the two molecules. A recombinant plasmid is then formed.