Microbiology: An Introduction, 11th Edition

Published by Benjamin Cummings
ISBN 10: 0321733606
ISBN 13: 978-0-32173-360-3

Chapter 3 - Observing Microorganisms Through a Microscope - Clinical Case - Question - Page 69: 1

Answer

The picture of the Gram stain shows that Gram-negative bacteria is present in the sample taken from the mucous lining of Maryanne's stomach. H. pylori, which is a Gram-negative bacterial strain, is still present.

Work Step by Step

1. Gram staining is a technique used to differentiate bacteria into Gram-positive bacteria and Gram-negative bacteria. 2. Gram-positive bacteria stains dark blue or purple as it retains the crystal violet dye after acetone-alcohol has been used. 3. Gram-negative bacteria stains red as it retains the counter-staining dye safranin. 4. The picture of the mucous lining sample from Maryanne's stomach shows red stained bacteria present. 5. The red staining indicates that the bacteria is Gram-negative. 6. The Gram stain was carried out to see if H. pylori is still present. 7. H. pylori is Gram-negative, so this would suggest that it is still present.
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