Campbell Biology (11th Edition)

Published by Pearson
ISBN 10: 0-13409-341-0
ISBN 13: 978-0-13409-341-3

Chapter 20 - Test Your Understanding - Level 3 - Synthesis/Evaluation - Page 439: 9


To clone the gene PCR could be used, choosing primers from the known sequence of the gene. The gene could be amplified and inserted in a plasmid using restriction enzymes. Depending on the length of the gene, more than one PCR product might be needed to be obtained and fitted together, again using restriction enzymes.

Work Step by Step

The key here is to see that the sequence of the gene is known. This allows for PCR, which is sequence-based, to be used. It is a quick and specific way to find a copy of the gene.
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