Campbell Biology (11th Edition)

Published by Pearson
ISBN 10: 0-13409-341-0
ISBN 13: 978-0-13409-341-3

Chapter 20 - 20.1 - Concept Check - Page 421: 2

Answer

- The other strand, the "complementary" strand would have the sequence: 3' - GGAACTGCTAGCAATGGC - '5 - This strand of DNA contains the restriciton enzyme site where PvuI can reate a double stranded break. The site is between the T and C nucleotides (designated by "^") in the sequence [1]: 5' - CGAT^CG - '3 3' - GC^TAGC- '5 [1] https://www.neb.com/products/r0150-pvui

Work Step by Step

- In DNA sequences, all nucleotides are matched to their complement on the opposite strand. To find the "complementary" strand match every nucleotide on the original strand with its complement, T to A and vice versa, and C to G and vice versa. - Ensure both strands are labeled the 5' and 3' end as to have both strands running anti-parallel to each other - Find the restriction site on both strands that matches the PvuI restriction enzyme site and insert a break. If no match is found, Pvul cant cut the DNA strand
Update this answer!

You can help us out by revising, improving and updating this answer.

Update this answer

After you claim an answer you’ll have 24 hours to send in a draft. An editor will review the submission and either publish your submission or provide feedback.