Othello

What proof does Iago give Othello about Desdemona's guilt?

Act 3

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Iago knows that Desdemona no longer has the handkerchief (Iago's wife gave it to him) and sets Othello up with the lie that Cassio has it. Othello will ask to see his precious handkerchief from Desdemona thus substantiating Iago's scheme.