act 1 and act 2
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L. Macbeth questions Macbeth's manhood, "When you durst do it, then you were a man; And to be more than what you were, you would Be so much more the man." She goes on to tell him to prove his love to her, "From this time / Such I account thy love". L. Macbeth spends much of this act goading her husband into doing the deed by praying on his male insecurities.
The most important thing lady macbeth does is convince Macbeth that he deserves to be king and that he should put all "this night's business" in her hands. When he hesitates, she calls him a chicken and puts out some pretty strong language about taking a child who was nursing her and dashing its brains out on the ground if she had said to him what he said to her. She convinces him that she will get Duncan's guards drunk and no one will question what has happened when there will be no one to give them away.