California Institute of Technology
What has been your most profound or surprising intellectual experience? or, Tell us about an experience that has changed the way you view yourself.
I arranged the bottles in front of me: 30% acrylamide, TEMED solution, ammonium persulfate, Tris buffer, and distilled water -- all of the materials I needed to run electrophoresis on the protein samples I had isolated earlier that morning. Oh, and isopropanol to even out the gel, I remembered. I reached for the bottle labeled “99% isopropanol,” but after angling it slightly, I realized that it was empty. I walked to the storage cabinets on the other end of the lab and peered inside. No isopropanol.
I found my mentor in the cell culture room, hunched over a microscope. Spraying antiseptic alcohol on my latex gloves, I hurried over.
“Dr. Wang, we’re out of isopropanol in the lab,” I announced after waiting several seconds.
Still staring into her culture medium, Dr. Wang replied, “Did you sterilize yourself before entering?”
“Yes, I did, with -- with the alcohol over there.” I pointed to a green plastic bottle that lay next to the electronic pipettes.
“The isopropanol is in the cabinets. I showed you yesterday,” Dr. Wang stated.
“I checked there and there’s no bottle of isopropanol. There’s just propanol, but--”
“Really? We’re out of isopropanol?” Dr. Wang finally looked up. “Remind me to place an order.” She returned to her cell...
Join Now to View Premium Content
GradeSaver provides access to 1228 study guide PDFs and quizzes, 9433 literature essays, 2428 sample college application essays, 424 lesson plans, and ad-free surfing in this premium content, “Members Only” section of the site! Membership includes a 10% discount on all editing orders.
Already a member? Log in