- Collegiate Assessor Kovalyov – the main character of the story is a civil servant of average rank. He is obsessed with his rank, and one day, he wakes up to find his nose missing.
- The Nose – this character is a body part that is personified in the story. By the way it is dressed, it seems to have achieved a higher rank of civil service than Kovalyov.
- Ivan Yakovlevitch – he is the barber who finds the nose in his bread. He attempts to throw the nose into the river. When the nose is miraculously reattached to Kovalyov again, he comes to Ivan to get shaved.
- Newspaper Advertising Clerk – he is who Kovalyov contacts to get an ad in the paper about his missing nose. When the newspaper advertising clerk first hears about the story, he is unable to understand what has happened. He rejects Kovalyov's ad because he believes that the ridiculousness of the story will make the newspaper look too sensational.
- Madame Podtochina – she is the mother of the girl that Kovalyov has been flirting with for some time. He refuses to propose to her because he believes he can marry someone even better, so Madame Podtochina is constantly bothering him about marrying her daughter.
- The doctor.
- The Police officer - he returns the lost nose back to Kovalyov.
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