- Machiavel, speaker of the Prologue
- Barabas, a rich Jewish merchant of Malta
- Abigail, his daughter
- Ithamore, his slave
- Ferneze, Governor of Malta
- Don Lodowick, his son
- Don Mathias, Lodowick's friend
- Katharine, Mathias' mother
- Friar Jacomo
- Friar Bernardine
- Abbess
- Selim Calymath, son of the Emperor of Turkey
- Callapine, a bashaw
- Martin del Bosco, Vice Admiral of Spain
- Bellamira, a courtesan
- Pilia-Borsa, her pimp
- Two merchants
- Three Jews
- A messenger
- A slave
- Knights of Malta
- Officers of Malta
- Bashaws serving the Turkish emperor
- Carpenters
- A nun
- Citizens of Malta, Turkish janizaries, guards, attendants, slaves
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