Verona:
- Valentine – a gentleman
- Proteus – Valentine's best friend
- Julia – Proteus' girlfriend
- Launce – Proteus' servant
- Speed – Valentine's servant
- Antonio – Proteus' father
- Panthino – Antonio's servant
- Lucetta – Julia's servant
- Crab – Launce's dog
Milan:
- The Duke – Silvia's father
- Silvia – Valentine's beloved
- Thurio – a foolish suitor to Silvia
- Sir Eglamour – a suitor to Silvia
The Forest:
- The Host – an innkeeper who helps Julia
- The Outlaws – banished men originally from Milan