Biology (11th Edition)

Published by McGraw-Hill Education
ISBN 10: 1-25918-813-2
ISBN 13: 978-1-25918-813-8

Chapter 25 - Evolution of Development - Figure 25.9 - Inquiry Question: 1

Answer

For functional analysis, ap3 mutants (lacking AP3 function) were transformed with either the functional AP3 gene or the functional gene minus its C terminus. The former construct restored function/petal formation, showing the AP3's function is to cause petals to form, and the version without the C terminus failed to to do this, showing that, for petal formation, the critical part of AP3 protein is the C terminus.

Work Step by Step

Functional analysis involves demonstrating gene function, usually by working with mutants that lack function and by restoring function in whole and in part using transgenes.
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