- In both Parts
- Tamburlaine – in Part 1 a Scythian shepherd; in Part 2 King of Persia
- Techelles – in Part 1 a follower of Tamburlaine; in Part 2 King of Fez
- Usumcasane – in Part 1 a follower of Tamburlaine; in Part 2 King of Morocco
- Theridamas – in Part 1 a Persian lord, later a follower of Tamburlaine; in Part 2 King of Algiers
- Zenocrate – daughter of the Soldan of Egypt; in Part 2 wife of Tamburlaine
- Only in Part 1
- Mycetes – King of Persia
- Cosroe – brother of Mycetes
- Ceneus – a Persian lord
- Ortygius – a Persian lord
- Meander – a Persian lord
- Menaphon – a Persian lord
- Bajazeth – Emperor of Turkey
- Zabina – wife of Bajazeth
- Ebea – maid of Zabina
- King of Algier
- King of Fez
- King of Morocco
- Alcidamus – King of Arabia
- Soldan of Egypt
- Governor of Damascus
- Agydas
- Magnetes[1]
- Capolin – an Egyptian
- Anippe - maid of Zenocrate
- Virgin of Damascus 1
- Virgin of Damascus 2
- Only in Part 2
- Calyphas – son of Tamburlaine
- Amyras – son of Tamburlaine
- Celebinus – son of Tamburlaine
- Orcanes – King of Natolia
- King of Jerusalem
- King of Soria
- King of Trebizon
- Gazellus – Viceroy of the city of Byron
- Uribassa
- Sigismund – King of Hungary
- Frederick – peer of Hungary
- Baldwin – peer of Hungary
- Callapine – son of Bajazeth
- Alameda – keeper of Callapine
- King of Amasia
- Governor of Babylon
- Captain of Balsera
- Son of the Captain of Balsera
- Olympia – wife of the Captain of Balsera
- Captain
- Maximus
- Peridicas
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