The work has been the subject of numerous scholarly writings, including detailed treatment in multiple works on Virginia Woolf. An "annotated" edition has been published to facilitate critical reading of the text.
The novel's title has also come to stand in some senses for women's writing generally, as one of the most famous works by a woman author that directly treats the subject of gender. For example, a project on the history of women's writing in the British Isles was named after the book.
The skating party on the Thames was featured in Simple Gifts, a Christmas collection of six animated shorts shown on PBS TV in 1977.
The novel has been adapted for theatre and film. In 1989 the American director, Robert Wilson, and writer Darryl Pinckney collaborated on a theatrical production. A British film adaptation was released in 1992, starring Tilda Swinton as Orlando and Quentin Crisp as Queen Elizabeth I. A second theatre adaptation by Sarah Ruhl premiered in New York 2010.
A similar character named Orlando, ageless and with varying sex and gender through the ages, is featured in Alan Moore and Kevin O'Neill's graphic novels The League of Extraordinary Gentlemen: Black Dossier, and The League of Extraordinary Gentlemen, Volume III: Century.