Rose wrote several stage adaptations of the story. In 1964, Leo Genn appeared in the play on the London stage, directed by Margaret Webster. In other theatrical adaptations in which female actors are cast the play is often retitled 12 Angry Jurors.
Harold Pinter directed a production of the play, which opened at the Bristol Old Vic on March 7, 1996. With set design by Eileen Diss, lighting design by Mick Hughes, and costume design by Tom Rand, its cast included Stuart Rayner (Juror 1, Foreman), Kevin Dignam (Juror 2), Tony Haygarth (Juror 3), Timothy West (Juror 4), Maurice Kaufmann (Juror 5), Douglas McFerran (Juror 6), Tim Healy (Juror 7), Kevin Whately (Juror 8), Alan MacNaughtan (Juror 9), Peter Vaughan (Juror 10), Robert East (Juror 11), Christopher Simon (Juror 12), Joshua Losey (Guard), and E. G. Marshall, as the Voice of the Judge.
The Roundabout Theatre Company presented a Broadway production of the play, starring Boyd Gaines as a more combative Juror 8, with James Rebhorn (Juror 4), Philip Bosco (Juror 3), and Robert Prosky as the Voice of the Judge.
In 2007, L.A. Theatre Works presented a production of the play that was recorded as an audiobook; directed by John de Lancie, the cast included Dan Castellaneta, Jeffrey Donovan, Hector Elizondo, Robert Foxworth, James Gleason, Kevin Kilner, Richard Kind, Alan Mandell, Rob Nagle, Armin Shimerman, Joe Spano, and Steve Vinovich.
The London West End production opened in November 2013 (running until 1 March 2014) at the Garrick Theatre. Directed by Christopher Haydon, the cast included Martin Shaw, Robert Vaughn, Jeff Fahey, Nick Moran, Robert Blythe, Miles Richardson and Martin Turner.
In 2014, Independent Theatre Company, Pakistan performed an adaptation of this play at Alhamra Arts Council, Lahore, directed by Azeem Hamid.