- Vindice, the revenger, frequently disguised as Piato (both the 1607 and 1608 printings render his name variously as Vendici, Vindic, and Vindice, with the latter spelling most frequent; in later literature Vendice[3]).
- Hippolito, Vindice's brother, sometimes called Carlo
- Castiza, their sister
- Gratiana, a widow, and mother of Vindice, Hippolito, and Castiza
- The Duke
- The Duchess, the duke's second wife
- Lussurioso, the duke's son from an earlier marriage, and his heir
- Spurio, the duke's second son, a bastard
- Ambitioso, the duchess's eldest son
- Supervacuo, the duchess's middle son
- Junior Brother, the duchess's third son
- Antonio, a discontented lord at the Duke's court
- Antonio's wife, raped by Junior Brother
- Piero, a discontented lord at the Duke's court
- Nobles, allies of Lussurioso
- Lords, followers of Antonio
- The Duke's gentlemen
- Two Judges
- Spurio's two Servants
- Four Officers
- A Prison-Keeper
- Dondolo, Castiza's servant
- Nencio and Sordido, Lussurioso's servants
- Ambitioso's henchman
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