Life: The Science of Biology 11th Edition

Published by W. H. Freeman
ISBN 10: 1-31901-016-4
ISBN 13: 978-1-31901-016-4

Chapter 18 - Recombinant DNA and Biotechnology - 18.2 - There Are Several Ways to Insert DNA into Cells - 18.2 Recap - Learning Outcomes - Page 386: 1


Plasmids are suitable for introducing new genetic material to cellular hosts because they are small and easily manipulated. In addition, the restriction enzyme recognition sequences of a plasmid provide an appropriate site for inserting foreign DNA.

Work Step by Step

Each fragment of separated DNA carries a reactive “overhang” at each cut. These overhangs are referred to as sticky ends because they have particular base sequences that are readily able to bind with complementary sticky ends.
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