- Lucrece – An honorable woman
- Collatine – Lucrece's husband, an officer in the Roman army
- Tarquin (Sextus Tarquinius) – Son of king Lucius Tarquinius and an officer in the Roman army, who rapes Lucrece
- Lucretius – Lucrece's father
- Junius Brutus – Friend to Collatine and Lucretius
- A Messenger
- Lucius Tarquinius (Tarquin the Proud) – King of Rome and Tarquin's father
- Servius Tullius – Father-in-law of Lucius Tarquinius
- Publius Valerius – Friend of Collatine and Lucretius
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