Mary Sidney was born on 18 October 1587 to the former Barbara Gamage (1563–1621), a wealthy Welsh heiress and first cousin once removed to Sir Walter Raleigh. Her father, Robert Sidney, 1st Earl of Leicester and Viscount Lisle of Penshurst Place, was a poet and governor of Flushing, Netherlands. Mary Wroth was niece to Mary Herbert née Sidney (Countess of Pembroke and one of the most distinguished women writers and patrons of the 16th century), and to Sir Philip Sidney, a famous Elizabethan poet-courtier.
Because her father, Robert Sidney, was governor of Flushing, Wroth spent much of her childhood at the home of Mary Sidney, and Penshurst Place, Baynard’s Castle in London. Penshurst Place was one of the great country houses in the Elizabethan and Jacobean period. It was a center of literary and cultural activity and its gracious hospitality is praised in Ben Jonson's famous poem To Penshurst. During a time when most women were illiterate, Wroth had the privilege of a formal education, which was obtained from household tutors under the guidance of her mother. With her family connections, a career at court was all but inevitable. Wroth danced before Queen Elizabeth on a visit to Penshurst and again in court in 1602. At this time a likeness of her as a girl in a group portrait of Lady Sidney and her children was painted by Marcus Gheeraerts the Younger in 1596, and is now on display at Penshurst. As a young woman, Lady Mary belonged to Queen Anne’s intimate circle of friends and actively participated in masques and entertainments.
On 27 September 1604, King James I married Mary to Sir Robert Wroth of Loughton Hall. The marriage was not happy; there were issues between the two beginning with difficulties over her father’s payment of her dowry. In a letter written to his wife, Sir Robert Sidney described different meetings with Robert Wroth, who was often distressed by the behavior of Mary shortly after their marriage. Robert Wroth appeared to have been a gambler, philanderer and a drunkard. More evidence of the unhappy union comes from poet and friend Ben Jonson, who noted that ‘my Lady Wroth is unworthily married on a Jealous husband’. Various letters from Lady Mary to Queen Anne also refer to the financial losses her husband had sustained during their time together.
During her marriage, Mary became known for her literary endeavours and also for her performances in several masques. In 1605 she danced at the Whitehall Banqueting House in The Masque of Blackness, which was designed by Ben Jonson and Inigo Jones. Mary Wroth joined the Queen and her friends in the production; all of whom painted their skin black to portray Ethiopian nymphs who called themselves the 'twelve daughters of Niger'. The masque was very successful and was the first in a long series of similar court entertainments. The ‘twelve daughters of Niger’ also appeared in The Masque of Beauty in 1608, also designed by Jonson and Jones. However, despite the success there were some less than favorable reviews, some referring to the women's portrayal of the daughters of Niger as ugly and unconvincing.
In February 1614 Mary gave birth to a son James: a month after this her husband Robert Wroth died of gangrene leaving Mary deeply in debt. Two years later Wroth's son died causing Mary to lose the Wroth estate to John Wroth, the next male heir to the entail. There is no evidence to suggest that Wroth was unfaithful to her husband, but after his death she entered a relationship with her cousin William Herbert, 3rd Earl of Pembroke. Mary and William shared interests in arts and literature and had been childhood friends. They had at least two illegitimate children, a daughter Catherine and son William. In "Herbertorum Prosapia", a seventeenth-century manuscript compilation of the history of the Herbert family (held at the Cardiff Library), Sir Thomas Herbert – a cousin of the earl of Pembroke – recorded William Herbert’s paternity of Wroth’s two children.
Mary Wroth’s alleged relationship with William Herbert and her children born from that union are referenced in her work, The Countess of Montgomery’s Urania. It is also claimed that William Herbert was a favorite of Queen Anne and that she is the reason he gained the position of the King's Lord Chamberlain in 1615. In Urania, Wroth repeatedly returns to references to a powerful and jealous Queen who exiles her weaker rival from the court in order to obtain her lover, causing many critics to believe this referenced tension between Queen Anne and Wroth over the love of Herbert.
The publication of the book in 1621 was a succès de scandale, as it was widely (and with some justification) viewed as a roman à clef. The diffuse plot is organized around relations between Pamphilia and her wandering lover, Amphilanthus, and most critics consider it to contain significant autobiographical elements. Although Wroth claimed that she never had any intention of publishing the book, she was heavily criticized by powerful noblemen for depicting their private lives under the guise of fiction. However, her period of notoriety was brief after the scandal aroused by these allusions in her romance; Urania was withdrawn from sale by December 1621. Two of the few authors to acknowledge this work were Ben Jonson and Edward Denny. Jonson, a friend and colleague of Mary Wroth praised both Wroth and her works in "Sonnet to the noble Lady, the Lady Mary Wroth." Jonson claims that copying Wroth’s works he not only became a better poet, but a better lover. Denny on the other hand provides a very negative critique of Wroth's work; he accused her of slander in a satiric poem, calling her a "hermaphrodite" and a "monster". While Wroth returned fire in a poem of her own, the notoriety of the episode may have contributed to her low profile in the last decades of her life. There was also a second half of Urania, which was published for the first time in 1999 and now resides in the Newberry Library in Chicago. According to Shelia T. Cavanaugh, the second portion of the work was never prepared by Wroth for actual publication and the narrative contains many inconsistencies and is somewhat difficult to read.
After the publication issues surrounding Urania, Wroth left King James's court and was later abandoned by William Herbert. There is little known about Wroth's later years but it is known that she continued to face major financial difficulties for the remainder of her life. Wroth died in either 1651 or 1653. Mary is commemorated in Loughton by the naming of a footpath adjacent to Loughton Hall as Lady Mary's Path.