- Ferdinand – King of Navarre
- Lord Berowne (or Biron), Lord Longueville (or Longaville) and Lord Dumaine – attending on the King
- Princess of France, later Queen of France
- Lady Rosaline, Lady Maria, Lady Katharine and Boyet – attending on the Princess
- Marcadé – messenger
- Don Adriano de Armado – a fantastical Spaniard
- Moth – Armado's page
- Sir Nathaniel – curate
- Holofernes – schoolmaster
- Dull – constable
- Costard – a rustic
- Jaquenetta – country wench
- Forester
- Officers and others, attendants on the King and Princess
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