The accusation of rape.
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Mayella accused Tom of rape in order to blame him when her father caught her attempting to seduce him.
To Kill a Mockingbird
So why does she say that? Was she trying to protect her dad from going to jail?
Mayella's attempted seduction of a black man would have been the worst of infractions. She did what her father told her to do, and she told the lies he forced her to tell.