The eyes were watching God
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There is the whole mule imagery directly linked with Janie's grandmother's statement that the "nigger woman is de mule uh de world"
There are also buzzards at the mule's funeral,
"The buzzards were holding a great flying-meet way up over the heads of the mourners and some of the nearby trees were already peopled with the stoop-shouldered forms."
There is of course the rabid dog. Tea Cake saves Janie from it but gets bitten and killed in the process.