The Royal Shakespeare Company staged a production of the play in 1996–1998, directed by Michael Boyd. The cast included Peter Wight as Hieronimo, Jeffry Wickham as the King of Spain, Paul Bentall as the Duke of Castille, Siobhan Redmond as Bel-imperia, and Deirdra Morris as Isabella.
An amateur production of The Spanish Tragedy was performed 2–6 June 2009 by students from Oxford University, in the second quad of Oriel College, Oxford. Another amateur production was presented by the Hyperion Shakespeare Company 21–30 October 2010 with students from Harvard University in Harvard's New College Theatre. In November 2012, Perchance Theatre in association with Cambridge University's Marlowe Society staged a site-specific production in King's College Chapel, Cambridge. In October/November 2013, the Baron's Men of Austin, TX performed the work in a near-uncut state, with period costumes and effects, at Richard Garriott's Curtain Theater, a mini replica of the Globe Theater. Another amateur production was presented by the Experimental Theater Board of Carleton College 27–29 May 2015.
Other professional performances include a modern-dress production staged at the Arcola Theatre in London in October–November 2009, directed by Mitchell Moreno, with Dominic Rowan as Hieronimo, as well as a production in Belle Époque era costume, staged by Theatre Pro Rata in Minneapolis in March 2010, directed by Carin Bratlie.
The play has never been filmed or staged on television.