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The division between man and animal is an important motif throughout this chapter. It primarily relates to Kino's descent from those human qualities he once displayed.
Steinbeck illustrates this through a number of events, such as when Kino attacks the trackers. In this instance, Kino moves from being capable of murder for self-defense to a more cold-blooded killing. Kino kills the three men out of fear and instinct and not because of any tangible threat they pose to him.
Steinbeck also shows the loss of human qualities within Kino when he crawls naked to find the trackers so that his white clothes will not expose him. He loses the final vestiges of humanity and society to become even more animalistic.