- Thomas
- The main protagonist of the novel. He is the last boy but not the last person to enter the Glade. The only thing he can remember when he comes into the Glade is his name, a common pattern amongst the Gladers. Chuck describes him as about 16 years old, of average height, and brown-haired. He is called "Greenie", a nickname given to new arrivals. He becomes a Runner with Minho after being the first person to spend a whole night (along with Minho) in the Maze and saves Alby when he is about to die.
- Teresa
- One of the main protagonists. The first girl and last person to enter the Glade. When she entered the Glade she was in a coma and Newt thought she was dead. She also calls Thomas "Tom". She is also known to help Thomas out of the maze and fight the Grievers in the Griever Hole. She is thin, has black hair and blue eyes, and relatively pale skin.
- Alby
- The eldest and the leader of the Gladers. He is described as a 'dark-skinned boy with short-cropped hair, his face clean-shaven'. He tries to keep order within the group by having all the boys follow the rules they've set down to survive. He has a very close relationship with Newt, his second-in-command. He was in the group of 30 people who first arrived in the Glade. Alby commits suicide by walking into a group of Grievers, thinking that it was better that he die there than outside the Maze.
- Newt
- One of the main protagonists and is good friends with Thomas and Minho. He used to be a Runner but is no longer able-footed since he was nearly attacked by a Griever. He is very kind, friendly, and welcoming to Thomas. He is Alby's closest friend and second-in-command and takes over as leader when Alby no longer feels capable. Newt was described as being rather 'tall and muscular', with blond hair that came down over his shoulders and a square jaw. He has a limp that he says is from running from a Griever, but the real reason is revealed to be an attempted suicide in the third book. In the books, Newt is often referred to as "the older boy", indicating that he is at least older than Thomas.
- Minho
- One of the main protagonists and is the Keeper of the Runners. He is in charge of navigating and mapping out the Maze. As a Runner, he is in very good shape and is described as "an Asian kid with strong, heavily-muscled arms and short black hair." He is sarcastic and a jokester. He tends to react without thinking, which leads him into trouble. He and Thomas quickly become good friends. He also has a girlfriend named Emily.
- Chuck
- A young and chubby boy with curly hair who was the newest Glader until Thomas arrived. He immediately becomes friends with Thomas and acts like a little brother towards him. Chuck is a "Slopper", one of the Gladers who handle all the dirty, distasteful jobs the others do not want. He is around 13 years old. He is killed by Gally after a dagger is thrown at Thomas and he saves him.
- Gally
- The main antagonist, Gally is a Glader who lives by the rules Alby put in place. He does not trust Thomas and shows an immense dislike for him. He is also the Keeper of the Builders. He runs away from the Glade in a fit of rage after exclaiming that he thought Thomas was not to be trusted in the Gathering. At the end of the book, he kills Chuck when he tries to save Thomas from being hit with a dagger.
- Ben
- A Builder. After undergoing the Changing and attempting to kill Thomas, he is banished to the Maze while still psychotic and dies overnight.
- Ava Paige
- The Chancellor of WICKED and the person responsible for sending teenagers into the Maze. She appears in the Epilogue in an e-mail.
- Grievers
- Biomechanical creatures that hunt and kill the Gladers in the maze. They are described as "large, bulbous creature[s] the size of a cow but with no distinct shape". In "The Ending", they are let into the Glade to kill one person every day.
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