The novel was adapted for the screen in 1940 with Margaret Lindsay as Hepzibah, George Sanders as Jaffrey, Vincent Price as Clifford, Dick Foran as Holgrave, and Nan Grey as Phoebe. In this adaptation, Hepzibah and Clifford were made lovers rather than brother and sister, and the film ends with a double wedding. Also, Clifford was well aware of Holgrave's true identity, and the two are working together to settle a score with Jaffrey. It was directed by Joe May with a screenplay by Lester Cole.
There was also a silent short in 1910 and a remake in 1967.
It was also loosely adapted as one of the three stories in the 1963 film Twice-Told Tales, along with "Rappaccini's Daughter" and "Dr. Heidegger's Experiment". All three sections featured Vincent Price.
The novel was adapted to a 60-minute television production in 1960 for The Shirley Temple Show with Shirley Temple as Phoebe, Robert Culp as Holgrave, Agnes Moorehead as Hepzibah, and Martin Landau as Clifford.