Answer
Due to the degeneracy of codons, one amino acid could have several different codons to match it, hence it is difficult to judge the mRNA sequence due to the protein sequence. Also, the position of ORF might be not clear, hence it will also affect the accuracy.
Work Step by Step
There are two sides to think about the answer: protein level and mRNA level. On the one hand, there may be degeneracy of amino acid codons, while on the other hand, the open reading frame may be not clear.
