Campbell Biology (10th Edition)

Published by Pearson
ISBN 10: 0321775651
ISBN 13: 978-0-32177-565-8

Chapter 20 - Test Your Understanding - Level 3 - Synthesis/Evaluation: 10

Answer

Please see the image.
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Work Step by Step

The goal in cloning is to get a useful copy of the gene in a vector, in this case the plasmid pictured in the question. It is important to realize that the copy of the gene needs to be inserted correctly. With identical cut ends the copy could be inserted in either proper or backwards orientation, so you need to include a technique to check the orientation of potential clones.
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