Campbell Biology (10th Edition)

Published by Pearson
ISBN 10: 0321775651
ISBN 13: 978-0-32177-565-8

Chapter 20 - 20.1 - Concept Check: 2

Answer

- The other strand, the "complementary" strand would have the sequence: 3' - GGAACTGCTAGCAATGGC - '5 - This strand of DNA contains the restriciton enzyme site where PvuI can reate a double stranded break. The site is between the T and C nucleotides (designated by "^") in the sequence [1]: 5' - CGAT^CG - '3 3' - GC^TAGC- '5 [1] https://www.neb.com/products/r0150-pvui
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Work Step by Step

- In DNA sequences, all nucleotides are matched to their complement on the opposite strand. To find the "complementary" strand match every nucleotide on the original strand with its complement, T to A and vice versa, and C to G and vice versa. - Ensure both strands are labeled the 5' and 3' end as to have both strands running anti-parallel to each other - Find the restriction site on both strands that matches the PvuI restriction enzyme site and insert a break. If no match is found, Pvul cant cut the DNA strand
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