Answer
1. remove plasmid from parent bacterium
2. use restriction enzymes to cut the DNA from bacterium and the animal/plant cell
3. use DNA ligase to stick DNA back together
4. introduce DNA into new host using a recombinant vector and clone the gene.
Work Step by Step
Recombinant DNA techniques have important applications in medicine, an example of which is manufacturing human insulin. Certain bacteria can also be genetically manipulated to consume oil spills.
