- Anthony Perkins as Norman Bates
- Vera Miles as Lila Crane
- John Gavin as Sam Loomis
- Martin Balsam as Private Investigator Milton Arbogast
- John McIntire as Deputy Sheriff Al Chambers
- Simon Oakland as Dr. Richmond
- Frank Albertson as Tom Cassidy
- Pat Hitchcock as Caroline
- Vaughn Taylor as George Lowery
- Lurene Tuttle as Mrs. Chambers
- John Anderson as California Charlie
- Mort Mills as Highway Patrol Officer
- Janet Leigh as Marion Crane
Virginia Gregg, Paul Jasmin, and Jeanette Nolan make uncredited appearances as the voice of Norma "Mother" Bates. The three voices were used interchangeably, except for the last speech, which was performed by Gregg.[13]
