Jim Burden: The narrator and protagonist of the novel, Jim grows up in Black Hawk, Nebraska and becomes a successful lawyer.
Josiah and Emmaline Burden: Jim's grandparents, who live on a farm in Nebraska.
Jake Marpole: Farm hand from Virginia at the Burden place.
Otto Fuchs: Farm hand from Austria at the Burden place.
Ántonia "Tony" Shimerda: The bold and free-hearted young Bohemian girl who moves with her family to Black Hawk, Nebraska.
Mr. and Mrs. Shimerda: Ántonia's immigrant parents from Bohemia.
Ambrosch, Marek and Yulka: Ántonia's brothers and sister.
Anton Cuzak: Ántonia's later husband.
Lena Lingard: Hired girl come from the countryside to work in Black Hawk.
Tiny Soderball: Hired girl who came from the countryside to work at the Gardener Hotel in Black Hawk.
Gaston Cleric: Jim's teacher in Lincoln at the University of Nebraska.
Minor characters include: Peter and Pavel, Ole Benson, The Cutters, Widow Steavens, Anton Jelinek.