The Athenians
- Theseus – Duke of Athens
- Hippolyta – Queen of the Amazons
- Hermia – in love with Lysander
- Helena – in love with Demetrius
- Lysander – in love with Hermia
- Demetrius – suitor to Hermia
- Egeus – Hermia's father
- Philostrate – Theseus' Master of Revels
The Mechanicals
- Peter Quince – a carpenter, their director and speaks the prologue
- Nick Bottom – a weaver, plays 'Pyramus'
- Francis Flute – a bellows-mender, plays 'Thisbe'
- Snug – a joiner, plays 'Lion'
- Tom Snout – a tinker, plays 'Wall'
- Robin Starveling – a tailor, plays 'Moonshine'
The Fairies
- Oberon – King of the Fairies
- Titania – Queen of the Fairies
- Puck – Oberon's knavish sprite
- A Fairy, Peaseblossom, Cobweb, Moth, Mustardseed – servants to Titania
- Indian changeling (unseen) – a ward of Titania
