- Morose: A gentleman that loves silence
- Sir Dauphine Eugenie: A Knight, Morose's nephew
- Ned Clerimont: A Gentleman, Dauphine's friend
- Truewit: Dauphine's other friend
- Epicoene: A young Gentlewoman, supposedly the silent woman
- Sir John Daw: A Knight, Epicoene's servant
- Sir Amorous la Foole: A Knight
- Thomas Otter: A land and sea Captain
- Cutbeard: A barber, also aids in tricking Morose
- Mute: One of Morose's servants
- Madame Haughty: Ladies Collegiates
- Madame Centaure, Ladies Collegiates
- Mistress Mavis, Ladies Collegiates
- Mistress Trusty, The Lady Haughty's woman
- Mistress Otter, The Captain's wife
- Parson
- Pages
- Servants
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