- Solinus – Duke of Ephesus
- Egeon – A merchant of Syracuse – father of the Antipholus twins
- Emilia – Antipholus' lost mother – wife to Egeon
- Antipholus of Ephesus and Antipholus of Syracuse – twin brothers, sons of Egeon and Emilia
- Dromio of Ephesus and Dromio of Syracuse – twin brothers, bondmen, each serving his respective Antipholus
- Adriana – wife of Antipholus of Ephesus
- Luciana – Adriana's sister, love interest of Antipholus of Syracuse
- Nell – kitchen wench/maid to Adriana, Wife of Dromio of Ephesus
- Luce - a witty and vivacious servant in the household of Antipholus of Syracuse
- Balthazar – a merchant
- Angelo – a goldsmith
- Courtesan
- First merchant – friend to Antipholus of Syracuse
- Second merchant – to whom Angelo is in debt
- Doctor Pinch – a conjuring schoolmaster
- Gaoler, Headsman, Officers, and other Attendants
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