Anne Killigrew excelled in multiple media, which was noted by contemporary poet, mentor, and family friend, John Dryden in his dedicatory ode to Killigrew. He addresses her as "the Accomplisht Young LADY Mrs Anne Killigrew, Excellent in the two Sister-Arts of Poësie, and Painting." Scholars believe that Kelligrew painted a total of 15 paintings; however, only four are known to exist today.
Many of her paintings display biblical and mythological imagery. Yet, Killigrew was also skilled at portraits, and her works include a self-portrait and a portrait of James, Duke of York. Some of her poetry references her own paintings, such as her poem "On a Picture Painted by her self, representing two Nimphs of DIANA's, one in a posture to Hunt, the other Batheing." Both her poems and her paintings place emphasis on women and nature, suggesting female rebellion in a male-dominated society. Contemporary critics noted her exceptional skill in both mediums, with John Dryden addressing his dedicatory