A Very Old Man With Enormous Wings

99. How did Gene Johnson culture the sample of Peter Cardinal’s blood serum? Describe the three steps of this experimental protocol.

the hot zone

Asked by
Last updated by jill d #170087
Answers 1
Add Yours

1) He placed the metal cylinder containing the virus into the Biosafety Level 4 cabinets.

2) "Next, he put his hands into the gloves, picked up the cylinder and, looking through a window to see what he was doing, peeled the tape off the cylinder."

"Inside it was a wad of paper towel soaked in bleach. He pulled apart the wad and found a Ziploc bag. It contained a couple of plastic tubes with screw tops. He unscrewed them and shook out two very small plastic vials containing golden liquid: Peter Cardinal's blood serum."

3) "Gene Johnson took a little bit of the boy's blood serum and dropped it into flasks that contained living cells from a monkey. If anything lived in Peter Cardinal's blood, it might begin to replicate in the monkey cells."

"Now for the next stage of the virus isolation. He drew off a little bit of fluid from the flasks and injected it into three rhesus monkeys, to infect them with the Cardinal agent."

"He took some of the Cardinal strain and injected it into guinea pigs to see if it would infect them. It killed them like flies."


The Hot Zone